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规格

规格	8样本、50样本(产品加服务)、22样本、8样本(产品加服务)、50样本、22样本(产品加服务)
样本类型	Cell Culture Supernatants, Plasma, Serum, Tissue Lysates, Other Body Fluids, Cell Lysates
原理方法	Sandwich-based
检测方法	Fluorescence Laser Scanner
定量与否	Quantitative
载体	Glass Slide
适用种属	Human
检测靶标	TNF alpha, bFGF, IGF-1, IL-17A, IL-6, PDGF-BB, IL-11, M-CSF, TGF beta 1, OPN, MMP-9, TGF beta 2, TGF beta 3, Activin A, Amphiregulin, BMP-2, BMP-4, BMP-6, BMP-7, BMP-9, DKK-1, E-Selectin, MMP-13, MMP-2, MMP-3, P-Cadherin, IL-8 / CXCL8, MIP-1 alpha / CCL3, aFGF / FGF-1, IL-1 beta / IL-1F2, VE-Cadherin / CDH5, TRANCE / TNFSF11, IL-1 alpha / IL-1F1, MCP-1 / CCL2, ICAM-1 / CD54, Osteoprotegerin / TNFRSF11B, Osteopontin / SPP1, Osteoactivin / GPNMB, RANK / TNFRSF11A, SDF-1 alpha / CXCL12 alpha, Sonic Hedgehog N-Terminal / Shh-N, VCAM-1 / CD106
靶标数	41

详细描述

Product Features

More cost-effective than traditional ELISA
High specificity and system reproducibility
Suitable for diverse sample types
Low sample volume requirement: 100 µL or less
Get results same day (6-hour processing time)
Well-suited for high throughput assays
Q Analyzer software provides one-step computation

Target Names

Scroll over each target protein for more information
Activin A Gene Symbol: INHBA Gene ID: 3624 Accession #: P08476 ELISA Kit: RayBio Human Activin A ELISA	aFGF (FGF-1) Gene Symbol: FGF1 Gene ID: 2246 Accession #: P05230 ELISA Kit: RayBio Human aFGF ELISA	Amphiregulin Gene Symbol: AREG Gene ID: 374 Accession #: P15514 ELISA Kit: RayBio Human AREG ELISA	bFGF Gene Symbol: FGF2 Gene ID: 2247 Accession #: P09038 ELISA Kit: RayBio Human bFGF ELISA	BMP-4 Gene Symbol: BMP4 Gene ID: 652 Accession #: P12644 ELISA Kit: RayBio Human BMP-4 ELISA
BMP-9 Gene Symbol: GDF2 Gene ID: 2658 Accession #: Q9UK05 ELISA Kit: RayBio Human BMP-9 ELISA	E-Selectin Gene Symbol: SELE Gene ID: 6401 Accession #: P16581 ELISA Kit: RayBio Human E-Selectin ELISA	ICAM-1 (CD54) Gene Symbol: ICAM1 Gene ID: 3383 Accession #: P05362 ELISA Kit: RayBio Human sICAM-1 ELISA	IGF-1 Gene Symbol: IGF1 Gene ID: 3479 Accession #: P05019 ELISA Kit: RayBio Human IGF-1 ELISA	IL-1 alpha (IL-1 F1) Gene Symbol: IL1A Gene ID: 3552 Accession #: P01583 ELISA Kit: RayBio Human IL-1 alpha ELISA
IL-1 beta (IL-1 F2) Gene Symbol: IL1B Gene ID: 3553 Accession #: P01584 ELISA Kit: RayBio Human IL-1 beta ELISA	IL-6 Gene Symbol: IL6 Gene ID: 3569 Accession #: P05231 ELISA Kit: RayBio Human IL-6 ELISA	IL-8 (CXCL8) Gene Symbol: IL8 Gene ID: 3576 Accession #: P10145 ELISA Kit: RayBio Human IL-8 ELISA	IL-11 Gene Symbol: IL11 Gene ID: 3589 Accession #: P20809 ELISA Kit: RayBio Human IL-11 ELISA	IL-17A Gene Symbol: IL17A Gene ID: 3605 Accession #: Q16552
MCP-1 (CCL2) Gene Symbol: CCL2 Gene ID: 6347 Accession #: P13500 ELISA Kit: RayBio Human MCP-1 ELISA	M-CSF Gene Symbol: CSF1 Gene ID: 1435 Accession #: P09603 ELISA Kit: RayBio Human M-CSF ELISA	MIP-1 alpha (CCL3) Gene Symbol: CCL3 Gene ID: 6348 Accession #: P10147 ELISA Kit: RayBio Human MIP-1 alpha ELISA	MMP-2 Gene Symbol: MMP2 Gene ID: 4313 Accession #: P08253 ELISA Kit: RayBio Human MMP-2 ELISA	MMP-9 Gene Symbol: MMP9 Gene ID: 4318 Accession #: P14780 ELISA Kit: RayBio Human MMP-9 ELISA
MMP-13 Gene Symbol: MMP13 Gene ID: 4322 Accession #: P45452 ELISA Kit: RayBio Human MMP-13 ELISA	Osteoactivin (GPNMB) Gene Symbol: GPNMB Gene ID: 10457 Accession #: Q14956 ELISA Kit: RayBio Human Osteoactivin ELISA	P-Cadherin Gene Symbol: CDH3 Gene ID: 1001 Accession #: P22223 ELISA Kit: RayBio Human P-Cadherin ELISA	RANK (TNFRSF11A) Gene Symbol: TNFRSF11A Gene ID: 8792 Accession #: Q9Y6Q6 ELISA Kit: RayBio Human RANK ELISA	SDF-1 alpha (CXCL12 alpha) Gene Symbol: CXCL12 Gene ID: 6387 Accession #: P48061 ELISA Kit: RayBio Human SDF-1 alpha ELISA
Sonic Hedgehog N-Terminal (Shh-N) Gene Symbol: SHH Gene ID: 6469 Accession #: Q15465 ELISA Kit: RayBio Human ShhN ELISA	TGF beta 1 Gene Symbol: TGFB1 Gene ID: 7040 Accession #: P01137 ELISA Kit: RayBio Human TGF-beta 1 ELISA	TGF beta 2 Gene Symbol: TGFB2 Gene ID: 7042 Accession #: P61812 ELISA Kit: RayBio Human TGF-beta 2 ELISA	TNF alpha Gene Symbol: TNF Gene ID: 7124 Accession #: P01375 ELISA Kit: RayBio Human TNF-alpha ELISA	VCAM-1 (CD106) Gene Symbol: VCAM1 Gene ID: 7412 Accession #: P19320 ELISA Kit: RayBio Human VCAM-1 ELISA
VE-Cadherin (CDH5) Gene Symbol: CDH5 Gene ID: 1003 Accession #: P33151	BMP-2 Gene Symbol: BMP2 Gene ID: 650 Accession #: P12643 ELISA Kit: RayBio Human BMP-2 ELISA	BMP-6 Gene Symbol: BMP6 Gene ID: 654 Accession #: P22004 ELISA Kit: RayBio Human BMP-6 ELISA	BMP-7 Gene Symbol: BMP7 Gene ID: 655 Accession #: P18075 ELISA Kit: RayBio Human BMP-7 ELISA	DKK-1 Gene Symbol: DKK1 Gene ID: 22943 Accession #: O94907 ELISA Kit: RayBio Human Dkk-1 ELISA
MMP-3 Gene Symbol: MMP3 Gene ID: 4314 Accession #: P08254 ELISA Kit: RayBio Human MMP-3 ELISA	Osteoprotegerin (TNFRSF11B) Gene Symbol: TNFRSF11B Gene ID: 4982 Accession #: O00300 ELISA Kit: RayBio Human OPG ELISA	Osteopontin (SPP1) Gene Symbol: SPP1 Gene ID: 6696 Accession #: P10451 ELISA Kit: RayBio Human OPN ELISA	PDGF-BB Gene Symbol: PDGFB Gene ID: 5155 Accession #: P01127 ELISA Kit: RayBio Human PDGF-BB ELISA	TGF beta 3 Gene Symbol: TGFB3 Gene ID: 7043 Accession #: P10600
TRANCE (TNFSF11) Gene Symbol: TNFSF11 Gene ID: 8600 Accession #: O14788

Application Notes

Suggested Application

Multiplexed Protein Detection; Biomarker Screening; Identifying Key Factors; Confirming Biological Process; Biomarker Validation; Validation of Antibody Array Results; Quantitative Protein Detection; Establishing Normal Range

Kit Components

Human Bone Metabolism Array Q1000 Slide(s)
Blocking Buffer
Wash Buffer 1
Wash Buffer 2
Lyophilized Standard Mix
Biotinylated Detection Antibody Cocktail
Streptavidin-Conjugated Fluor
Slide Washer/Dryer
Adhesive Plastic Strips
Manual

Other Materials Required

Distilled or deionized water
Small plastic boxes or containers
Pipettors, pipette tips and other common lab consumables
Orbital shaker or oscillating rocker
Aluminum foil
Gene microarray scanner or similar laser fluorescence scanner

View Compatible Laser Scanners

Don't have a compatible scanner? RayBiotech now offers FREE scanning service for all RayBio glass slide antibody arrays! Learn More

Protocol Outline

Dry the glass slide
Prepare Standards
Block array surface
Incubate with Samples and Standards
Incubate with Biotinylated Detection Antibody Cocktail
Incubate with Streptavidin-Conjugated Fluor
Disassemble the glass slide
Scan with a gene microarray laser scanner
Perform densitometry and analysis

Storage/Stability

For best results, store the entire kit frozen at -20°C upon arrival. Stored frozen, the kit will be stable for at least 6 months which is the duration of the product warranty period. Once thawed, store array slide(s), standard mix, detection antibody cocktail, and Cy3-Conjugated Streptavidin at -20°C and all other reagents undiluted at 4°C for no more than 3 months.